Resources

BIU offers a state-of-the-art optical imaging infrastructure that is unique in Greece.

Confocal Leica TCS SP5 on a DMI6000 Inverted Microscope
Confocal Leica TCS SP5 II  on a DM 6000 CFS Upright Microscope
Quantitative Photometry and Imaging system - PTI Ratio/Image Master
Neurolucida system for Neuroanatomical Analysis – MBF Bioscience
Stereo Investigator system for Stereology – MBF Bioscience
Digital Imaging Microscopes
Computing
Software

Confocal Leica TCS SP5 on a DMI6000 Inverted Microscope

The confocal is a Leica TCS SP5 with the dual (Tandem) Scanner.

The Tandem Scanner combines two options in one system: a conventional, slow (1000 lines/sec) but accurate scanning system - ideal for morphological imaging and three dimensional structure analyses, and a resonant scanning system (8000 lines/sec) - ideal for high speed confocal imaging. Laser lines at 405, 458, 476, 488, 496, 514, 561, 594 and 633nm. FRET and FRAP capability. Environment control enclosure (for temperature and CO2 control) Fully automated stage in all 3 dimensions xyz for tilling experiments Long term, multi-colour, multi-location time-lapse experiment capability.

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Confocal Leica TCS SP5 II on a DM 6000 CFS Upright Microscope

The fixed stage microscope DM 6000 facilitates the imaging of tissue slices, whole organs and small embryos. It will soon be upgraded with a 405nm Laser for DAPI imaging. Laser lines at 458, 476, 488, 496, 514, 561, and 633nm. FRET and FRAP wizards Fast z-stage for real time XZ or YZ sectioning Fully automated stage in all 3 dimensions xyz Long term, multi-colour, multi-location time-lapse experiment capability.

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Quantitative Photometry and Imaging system - PTI Ratio/Image Master

A combination of a dual channel photometry (up to 2KHz sampling rate) and imaging system (intensified CCD), for quantitative fluorescence microscopy of live cells from PTI - Photon Technology International

Specifications:

• Dual emission channel photometers
• Intensified CCD camera (PTI-IC200) with the Image Master software package.
• DeltaRAM X, high speed, random access monochromator.
• Automatic live-cell edge detection system

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Neurolucida system for Neuroanatomical Analysis – MBF Bioscience

On a upright Leica microscope with a high-resolution motorized stage and a colour camera (Retiga 2000 - Q Imaging). For tracing, 3D mapping, Image analysis and morphometry of neurons filled with either fluorescent indicators or coloured stains.  Data can be traced on-line from the attached microscope or imported as confocal stacks.

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Stereo Investigator system for Stereology – MBF Bioscience

On a upright Leica microscope with a high-resolution motorized stage and a colour camera (Retiga 2000 - Q Imaging). For the un-biased quantification of cell counts, lengths, areas and volumes and other morphological properties of 3D structures from 2D data.  Data can be acquired either directly from the attached microscope or imported as confocal stacks.

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Digital Imaging Microscopes

Two upright Leica (DMRA2), two inverted Leica (DMIRE2) microscopes and a fluorescence stereoscope (NIKON - SMZ800) with digital cameras and slide scanner for high resolution fluorescence or bright field imaging.

Cameras:

• Leica DFC-500 (CCD-Colour 12Mpixel)
• Q Imaging Retiga 2000 (CCD-Colour 5Mpixel)
• Hamamatsu ORCA-Flash4.0 V2 (sCMOS Monochrome - 4Mpixel)
• Hamamatsu ORCA-Flash4.0 LT (sCMOS Monochrome - 4Mpixel)
• PrimeHisto XE whole slice scanner for up to 10.000dpi resolution

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Computing

BIU incorporates an image analysis suite equipped with computers with specialised hardware and Image analysis software packages. All computers are linked via a fast (Gbit) network to two NAS servers (RAID5) for safe, unified storage.

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