BRFAA - Biomedical Research Foundation Academy Of Athens
Biomedical Research Foundation Academy Of AthensAcademy Of Athens


Leica Stellaris 8 Dive/Falcon
Multi-photon/FLIM Confocal Microscope


Deep imaging of thick samples through multi-photon microscopy complemented by lifetime (FLIM) measurements. Single photon confocal microscopy is also available.


  • Upright fixed-stage confocal microscope, with inserts for slides and small animals
  • Single Photon excitation: Diode Lasers 405nm, 448nm, 488nm, 561nm, 638nm
  • 2P/3P excitation: SpectraPhysics InSight Tunable, dual line multi-photon laser. Fixed line @ 1045nm, tuneable line @ 700nm – 1300nm
  • 2nd harmonic imaging
  • Specialised objectives:
    • 10/0.4 PLAN APO dry objective for tiling and navigation
    • 16/0.8 IMM motCORR, for dipping into cleared specimens
    • 25/0.95NA IRAPO - InfraRed corrected. Deep tissue imaging under coverslip
    • 40/1.25 glycerol for high resolution confocal imaging
    • 63/1.4 oil for high resolution confocal imaging.
  • Five very sensitive, photon counting HyD detectors (3 internal-2 External)
  • 4Tune - spectrally tuneable, multi-photon detection unit, providing high flexibility and enabling multi-color deep in vivo experiments – With 3 non-descanned Detectors.
  • NAVIGATOR - Multi-position - Tile scan module for big sample overviews
  • LIGHTNING – Real-time, deconvolution based, super-resolution module
  • FALCON - LifeTime Module with 2 detectors for FRET measurements and τ-sense Imaging
  • FRAP/Cell Ablation module
  • Temperature Control enclosure (with both cooling and heating capability)

The System was funded partially by the project “Innovational Multi-Vision, Multi-photon Laser Microscopy System for Advanced 3D Diagnostic Applications and Preclinical Studies” (Dr. D. Mangoura, MIS 5060306) funded partly by the Regional Operational Programme "Attica" (NSRF 2014-2020) co-financed by Greece and the European Union (European Regional Development Fund) and partly by Biomedical Research Foundation of the Academy of Athens.