BRFAA - Biomedical Research Foundation Academy Of Athens
Biomedical Research Foundation Academy Of AthensAcademy Of Athens


Our primary focus is to provide help and consultation to BRFAA investigators. However the unit is also open to external users (for a fee) and/or collaborators. BIU scientific personnel have many years of research experience in optical microscopy and the application of imaging technology to biology and medicine. Assistance for projects requiring intensive image processing and analysis is provided through scientific collaborations.  Please contact us for more information.

Microscopy techniques

  • Confocal Microscopy
  • Wide-field microscopy
  • Deconvolution Microscopy
  • Structured Illumination Microscopy (SIM)
  • FRAP/FLIP (Photo-bleaching Experiments)
  • FRET (Energy Transfer Experiments)
  • Live cell Imaging with temperature and CO2 regulation
  • Quantitative Ion Imaging (Calcium or pH Imaging)
  • Colour Brightfield Imaging
  • Whole-slide colour scanning
  • Neuroanatomical Tracing (Neurolucida)
  • Unbiased Stereology (Stereo Investigator)

Image Processing and Analysis

  • 3D Visualisation and Measurements (Imaris, Volocity)
  • Probe co-localisation
  • Multi-Fluorescent Protein detection (Spectral Unmixing)
  • Cell tracking
  • Particle Image Velocimetry (PIV)
  • Machine Learning based segmentation (Ilastic)
  • Image Restoration software
  • Custom Image Processing and Analysis software (ImageJ)