Biomedical Research Foundation Academy Of AthensAcademy Of Athens
Scientific Personnel

Aristidis S. Aristidis S. Charonis, MD, PhD
Investigator - Professor Level

Telephone : +30 210 6597 205
Fax : +30 210 6597 545
e-mail : acharonis@bioacademy.gr


Center :

Clinical, Experimental Surgery & Translational Research

Lab Site :

Charonis Lab


Brief Bio

Aristidis S. Charonis was born in Athens, Greece, in 1952. He graduated from Varvakion High School and attended the Medical School of the University of Athens, where he received his M.D. degree in 1976. He received his Ph.D. in Cell Biology in 1982 from the University of California San Francisco. He did postdoctoral work at Yale University Medical School and from 1987 till 1998 he was a faculty member (Assistant and Associate Professor with tenure) at the Department of Pathology, University of Minnesota Medical School. From 1995 till 2002 he was an Associate Professor of Histology at the University of Patras Medical School. From 2002 he is a Chief Researcher at the Biomedical Research Foundation of the Academy of Athens. He is also involved in the effort to coordinate biobanking in Greece and to connect the Greek biobanks with the European network, being recently built as a major modern infrastructure.

Dr. Charonis's early work focused on the structure and function of extracellular matrix macromolecules in health and disease. His early work focused on laminin, studying its properties of self-assembly and interaction with other extracellular macromolecules and cell surface receptors and mapping interaction sites. Diabetic modifications of laminin and kidney basement membranes were also studied, along with their influence in cellular phenotype. He later worked on tubulointerstitial nephritis antigen (TIN-ag) a basement membrane macromolecule expressed almost exclusively in the kidney. His current main focus is on renal diseases with emphasis on the fibrotic process. Using proteomic approaches he is exploring new macromolecules involved in renal fibrosis, trying to decipher their role, the pathways that are participating in and the possibility to be used as early markers of fibrosis; cell culture studies, animal models and biopsy material are all used in these studies. These studies focus on calreticulin and transgelin and the family of 14-3-3 proteins. The molecular mechanisms involved in their up-regulation are being studied. Also, renal biopsy material and urine samples are being studied, in order to confirm the up-regulation of these proteins in various nephropathies.

In addition, his scientific interest includes the patho-physiology of pancreatic beta cells. Using proteomic approaches, he has identified that ERp46, a protein compnent of the endoplasmic reticulum, is involved in diabetic conditions and he is exploring its role in insulin production.

Selected Publications

Charonis AS, Skubitz APN, Koliakos GG, Reger LA, Dege J, Vogel AM, Wohlhueter R and Furcht LT (1988). A novel synthetic peptide from the B1 chain of laminin with heparin-binding and cell adhesion-promoting activities. J.Cell Biol. 107, 1253-1260

Anderson SS, Tsilibary EC and Charonis AS (1993). Nonenzymatic glycosylation induced modifications of intact tubular basement membrane. J.Clinical Invest. 92, 3045-3052

Hasegawa G, Hunter AJ and Charonis AS (1995). Matrix nonenzymatic glycosylation leads to altered cellular phenotype and intracellular tyrosine phosphorylation. J.Biol. Chem. 270, 3278-3283

Chen Y, Krishnamurti U, Wayner E, Michael A and Charonis AS (1996). Receptors in proximal tubule epithelial cells for tubulointerstitial nephritis antigen (TIN-ag). Kidney Internat. 49, 153-157

Zhou B, Nelson TR, Kashtan C, Gleason B,Vlassi M, Michael AF and Charonis AS (2000). Identification of two alterantively spliced forms of human tubulointerstitial nephritis antigen (TIN-ag). Journal of the American Society of Nephrology 11, 658-68.

Kypreou K, Kavvadas P, Karamessinis P, Peroulis M, Alberti A, Sideras P, Psarras S, Capetanaki Y, Politis P, Charonis AS (2008). Altered expression of calreticulin during the development of fibrosis. Proteomics, 8, 2407-2419

Alberti A, Karamessinis P, Peroulis M, Kypreou K, Kavvadas P, Pagakis S, Politis P, Charonis AS (2009) ERp46 is reduced by high glucose and regulates insulin content in pancreatic beta cells. Amer. J. Physiol. Endocr.-Metab, 297:E812-E821

Charonis A, Luider T, Baumann M, Schanstra JP (2011) Viewpoint: is the time ripe for kidney tissue proteomics?  Proteomics: Clinical Applications, 5:215-221

Klein J, Kavvadas P, Prakoura N, Karagianni F, Schanstra JP, Bascands JL, Charonis AS (2011) Renal fibrosis: Insight from proteomics in animal models and human disease Proteomics, 11:805-815

Karagianni F, Prakoura N, Kaltsa G, Politis P, Arvaniti E, Kaltezioti V, Psarras S, Pagakis S, Katsimboulas M, Abed A, Chatziantoniou C, Charonis A. Transgelin Up-Regulation in Obstructive Nephropathy.  PLoS One. 2013 Jun 26;8(6):e66887. Print 2013.

Assimakopoulos SF, Charonis AS.  Uncovering the molecular events associated with increased intestinal permeability in liver cirrhosis: the pivotal role of enterocyte tight junctions and future perspectives.  J Hepatol. 2013 Nov;59(5):1144-6

Prakoura N, Politis PK, Ihara Y, Michalak M, Charonis AS.  Epithelial calreticulin up-regulation promotes profibrotic responses and tubulointerstitial fibrosis development.   Am J Pathol. 2013 Nov;183(5):1474-87

PubMed:

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