| Transgenic Mice |  | | |
The Transgenic Mouse Facility at the Biomedical Research Foundation of the Academy of Athens provides services which includes microinjection of oocytes and transplantation of embryos into foster recipients for the generation of transgenic mice. ES cell injections and blastocyst transplantation to generate knockout mice is also covered. Assistance is given to all investigators for their transgenic related project functions, which include construct design, DNA preparation, mouse handling, genotyping, and colony generation.
Services
The Transgenic Mouse Facility supports the research activities of the Biomedical Research Foundation by providing:
- Mouse handling and breeding
- Transgenic construct design
- Preparation of DNA for microinjection
- Preparation of PCR quality DNA from tail biopsies
- Re-derivation of infected transgenic and knock-out mouse lines
- Microinjection of oocytes and transplantation of embryos into foster
- recipients for the generation of transgenic mice
- ES cell injections and blastocyst transplantation to generate knockout mice (soon)
Infrastructure
The transgenic facility at the Biomedical Research Foundation of the Academy of Athens is located within a barrier (SPF) on the lower floor of the central Research Building of the Biomedical Research Foundation.
Major equipment in the transgenic core consists of the following:
- One dissecting microscope for embryo recovery and embryo transfer
- Programmable pipette puller
- micropipette grinder
- microforge
- One CO2 incubator
- One complete microinjection station containing a Leica inverted microscope on an anti-vibrating table; two Eppendorf-Leica micromanipulators, a pneumatic Eppendorf microinjector for DNA injection into one-cell embryos as well as manual injector for injecting stem cells into blastocysts
Spiros Georgopoulos Ph.D.
Investigator - Assistant Professor Level
Cellular Biology Division
Center of Basic Research I
Telephone: +30 210 6597187
Fax: +30 210 6596545
Email: sgeorgopoulos  bioacademy.gr
| 
